| Chukwudi Iwuji | .... | The High Evolutionary |
| Bradley Cooper | .... | Rocket (Stimme) |
| Pom Klementieff | .... | Mantis |
| Dave Bautista | .... | Drax |
| Karen Gillan | .... | Nebula |
| Vin Diesel | .... | Groot (Stimme) |
| Austin Freeman | .... | On-Set Groot / Phlektik Guard |
| Stephen Blackehart | .... | Steemie Blueliver |
| Terence Rosemore | .... | Xlomo Smeth |
| Maria Bakalova | .... | Cosmo (Stimme) |
| Sean Gunn | .... | Kraglin / Young Rocket |
| Sarah Alami | .... | Ssssaralami |
| Jasmine Munoz | .... | Hoobtoe |
| Chris Pratt | .... | Peter Quill / Star-Lord |
| Giovannie Cruz | .... | Orloni Peddler |
| Will Poulter | .... | Adam Warlock |
| Nico Santos | .... | Recorder Theel |
| Miriam Shor | .... | Recorder Vim |
| Noa Raskin | .... | Baby Rocket (Stimme) |
| Linda Cardellini | .... | Lylla (Stimme) |
| Asim Chaudhry | .... | Teefs (Stimme) |
| Mikaela Hoover | .... | Floor (Stimme) |
| Elizabeth Debicki | .... | Ayesha |
| Judy Greer | .... | War Pig (Stimme) |
| Reinaldo Faberlle | .... | Behemoth (Stimme) |
| Sylvester Stallone | .... | Stakar Ogord |
| Tara Strong | .... | Mainframe (Stimme) |
| Jared Gore | .... | Krugarr |
| Michael Rosenbaum | .... | Martinex |
| Elan Gale | .... | Beardy Ravager |
| Molly Quinn | .... | Molly Ravager |
| Gerardo Davila | .... | Fitz-Gibbonok |
| Dee Bradley Baker | .... | Blurp |
| Zoe Saldaņa | .... | Gamora |
| Hanna Pak | .... | Humanimal Turtle Experiment |
| Jennifer Holland | .... | Administrator Kwol |
| Nathan Fillion | .... | Master Karja |
| Kyle McLean | .... | The Boss's Nephew |
| Benjamin Byron Davis | .... | Bletelsnort |
| Tiffany Smith | .... | Orgocorp Host Friniki |
| Joe Daru | .... | Security Assistant |
| Daniela Melchior | .... | Ura |
| Jonathan Mercedes | .... | Gamora Shoots This Guy! |
| Jonathan Fritschi | .... | Carrot Head Guard |
| Diego Ward | .... | Carrot Head's Friend |
| Max Bickelhaup | .... | Kitty Orgosentry |
| Brandon Morales | .... | Dancing Guard |
| Renae Moneymaker | .... | Violent Orgosentry |
| Alexis Hadesty | .... | Humanimal Rabbit |
| Tara Warren | .... | Humanimal Kangaroo |
| Candi VandiZandi | .... | Humanimal Monkey |
| Grecia Balboa | .... | Warthog Mother |
| Caleb Spillyards | .... | Humanimal Panda Bear |
| Darla Delgado | .... | Neelie |
| Michelle Civile | .... | Blue Jay Postal Worker |
| Ken Lyle | .... | Rabbit Businessman |
| Elodie Clarke | .... | Humanimal Bush Baby Toddler |
| John William Wright | .... | Humanimal Hyena |
| Autumn Griffin | .... | Humanimal Bat Child |
| Skylar Huntley | .... | Humanimal Bat Child |
| Randy Havens | .... | Till |
| Dane Diliegro | .... | Unsavory Octopus |
| Kai Zen | .... | Phyla |
| a different name | .... | Latti |
| Yael Ocasio | .... | Star Child |
| Adelynn Spoon | .... | Star Child |
| Henry Heffernan | .... | Star Child |
| Brooklyn Skye Oliver | .... | Star Child |
| Scarlett Blum | .... | Star Child |
| Baby Dro | .... | Star Child |
| Emery Grilliot | .... | Star Child |
| Amelia Waters | .... | Star Child |
| Lloyd Kaufman | .... | Gridlemop |
| Seth Green | .... | Howard the Duck (Stimme) |
| Christopher Fairbank | .... | Broker |
| Rhett Miller | .... | Bzermikitokolok |
| Natalia Safran | .... | Controller |
| Murphy Weed | .... | Murf |
| Michael Rooker | .... | Yondu |
| James Gunn | .... | Lamb-Shank |
| Pete Davidson | .... | Phlektik |
| Jessica Fontaine | .... | Phlektik Guard |
| Karen Abercrombie | .... | Grandma Quill |
| Gregg Henry | .... | Grandpa Quill |
| Adriana Leonard | .... | Crying Krylorian Citizen |
| Bonnie Discepolo | .... | Crying Krylorian Citizen |
| Clare Grant | .... | Crying Krylorian Citizen |
| Grace Gunn | .... | Crying Krylorian Citizen |
| Will Gunn | .... | Crying Krylorian Citizen |
| Sarah Allyn | .... | Homeless Sad Rabbit (uncredited) |
| Thomas R. Baker | .... | Villager / Sentry (uncredited) |
| Jerry Beharry | .... | Dark (uncredited) |
| R. Grayson Brice | .... | Knowhere Citizen Blue Alien (uncredited) |
| James Clarke | .... | Knowhere Citizen (uncredited) |
| Finn David | .... | Star Kid (uncredited) |
| Sarah Dean | .... | Knowhere Kree (uncredited) |
| Jose Degracia | .... | Knowhere Citizen (uncredited) |
| Mark Joseph Felton | .... | Rabbit Humanimal (uncredited) |
| Gregory French | .... | Recorder Guard (uncredited) |
| Christina R Gregg | .... | Alien Ravager / Human Knowhere Citizen (uncredited) |
| Rowan Delana Howard | .... | Star Child (uncredited) |
| Elbert Kim | .... | Recorder Scientist (uncredited) |
| Michael Koske | .... | Naked Mole Rat (uncredited) |
| Aaliyah Luu | .... | Star Child (uncredited) |
| C.J. Maxwell | .... | Red Ravager #1 (uncredited) |
| Christina Mayo | .... | Orgocorp Xeronian (uncredited) |
| Andrew S. McMillan | .... | Recorder Scientist (uncredited) |
| Zachary Ortega | .... | Star Kid (uncredited) |
| Jalen Peet | .... | Scientist Recorder (uncredited) |
| Olive Raine | .... | Star Kid (uncredited) |
| Dennice Rivera | .... | Club Patron / Spaceport Worker (uncredited) |
| Rory Ross | .... | Krylorian villager (uncredited) |
| Casey Shirley | .... | Knowhere Resident (uncredited) |
| Sebastian Soler | .... | Checkers (uncredited) |
| Liam Stuckey | .... | Star Child (uncredited) |
| Juan Szilagyi | .... | Alien Kree (blue skin) (uncredited) |
| Analisa Wall | .... | Knowhere Dancer (uncredited) |
| Ilara Phoenix Williams | .... | Star Child (uncredited) |